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ASAP-modules

ASAP is a set of modules to perform stitching and alignment of EM and Array tomography data. It is suitable for processing large-scale datasets and supports multiple computational environments.

Installation

Please refer the documentation Installation:Installation. on how to install and use ASAP modules

How to run

The order of processing is as follows;

  1. Lens distortion correction

  2. Mipmap generation

  3. Montaging and Montage QC

  4. Global 3D non-linear alignment

Other Modules

A few other modules are included in ASAP to do the following.

  1. Materialization - render intermediate/final aligned volume to disk for further processing

  2. Fusion - Fuse global 3D non-linear aligned chunks together to make a complete volume

  3. Point match filter - A module that performs point match filtering of an existing point match collection

  4. Point match optimization - Performs a parameter sweep from a given set of ranges on a random sample of tilepairs to identify the optimal set of parameters

  5. Registration - Register individual sections in an already aligned volume (useful in cases of aligning missing/reimaged sections)

Support

We are planning on occasional updating this tool with no fixed schedule. Community involvement is encouraged through both issues and pull requests. Please make pull requests against the dev branch, as we will test changes there before merging into master.

Acknowledgments

This project is supported by the Intelligence Advanced Research Projects Activity (IARPA) via Department of Interior / Interior Business Center (DoI/IBC) contract number D16PC00004. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright annotation theron.

Disclaimer: The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of IARPA, DoI/IBC, or the U.S. Government.